| # | Swimmer | Team | Seed | Time | Diff |
| 1 |
Petersen, Amanda K (16)
|
CAROSE |
53.29 |
51.54 |
-1.75 |
| 2 |
Donald, Meghan A (15)
|
IAACAC |
53.44 |
51.88 |
-1.56 |
| 3 |
Young, Georgia G (17)
|
CAALPH |
51.60 |
51.94 |
0.34 |
| 4 |
Djunic, Helena (16)
|
UTCITY |
52.06 |
52.27 |
0.21 |
| 5 |
Waterson, Rebecca Q (17)
|
SNDART |
51.82 |
52.38 |
0.56 |
| 6 |
Brede, Chloe E (15)
|
OZSEA |
52.71 |
52.63 |
-0.08 |
| 7 |
Whitaker, Ava G (15)
|
NTRACE |
53.45 |
52.66 |
-0.79 |
| 8 |
Brock, Elizabeth H (16)
|
COSTAR |
53.13 |
52.67 |
-0.46 |
| 9 |
Gewalt, Eleni K (15)
|
WIPX3 |
52.63 |
52.68 |
0.05 |
| 10 |
Sanchez, Alexandra L (15)
|
COTOPS |
54.01 |
52.98 |
-1.03 |
| 11 |
Christian, Kate M (14)
|
PCDA |
52.61 |
53.15 |
0.54 |
| 12 |
Shaw, Abigail C (18)
|
COEA |
53.04 |
53.34 |
0.30 |
| 13 |
Bluford, Kaitlyn L (14)
|
AZMAC |
55.00 |
54.14 |
-0.86 |
|
Ciezczak, Megan A (16)
|
ILFOX |
51.49 |
NS |
- |
|
Schuricht, Blakely R (15)
|
AZSAC |
56.68 |
NS |
- |